Production of monoclonal antibodies based on the CIPEX expression system

The production organism Tetrahymena secretes expressed proteins with a uniform and consistent biantennary oligomannose glycosylation structure and might have a positive influence on the effector function ADCC of monoclonal antibodies.

Demonstration of a monoclonal antibody (IgG2b) produced in Tetrahymena.

A: Silver stained SDS-Gel
B: Coomassie stained Gel (after purification from supernatant of a Tetrahymena culture)
C: Immunoblot (after purification from supernatant of a Tetrahymena culture)






Confirmation of the specificity of the monoclonal antibody produced in Tetrahymena using FACS-analysis.

The antibody produced in Tetrahymena detects as much CD4CD3-double positive T-cells as a comparable antibody.

The research was granted by the BMBF and BMWi (ZIM).




The market for monoclonal antibodies is the fastest growing segment of the pharmaceutical industry. It now accounts for about 1/3 of sales of a 45 billion euro market for biopharmaceuticals, and rising.