Production of monoclonal antibodies based on the CIPEX expression system
The production organism Tetrahymena secretes expressed proteins with a uniform and consistent biantennary oligomannose glycosylation structure and might have a positive influence on the effector function ADCC of monoclonal antibodies.
Demonstration of a monoclonal antibody (IgG2b) produced in Tetrahymena.
A: Silver stained SDS-Gel
B: Coomassie stained Gel (after purification from supernatant of a Tetrahymena culture)
C: Immunoblot (after purification from supernatant of a Tetrahymena culture)
Confirmation of the specificity of the monoclonal antibody produced in Tetrahymena using FACS-analysis.
The antibody produced in Tetrahymena detects as much CD4CD3-double positive T-cells as a comparable antibody.
The research was granted by the BMBF and BMWi (ZIM).
€The market for monoclonal antibodies is the fastest growing segment of the pharmaceutical industry. It now accounts for about 1/3 of sales of a 45 billion euro market for biopharmaceuticals, and rising.